Figure 8. Insulin signalling activates the TGF‐ß inhibitor Sog.

• A–D′
  Overexpression of mCherry ^RNAi , InR ^CA ; mCherry ^RNAi , Sog ^RNAi ; mCherry ^RNAi and Sog ^RNAi ;InR ^CA under the control of r4‐GAL4 stained for pMad and DAPI. The same mCherry ^RNAi sample was used in Fig 7M, as these experiments were carried out together).
• E
  Quantification of pMad levels of (A–D′). mCherry ^RNAi (n = 17), InR ^CA ; mCherry ^RNAi (n = 7), Sog ^RNAi ; mCherry ^RNAi (n = 26), and Sog ^RNAi ;InR ^CA (n = 19), The same mCherry ^RNAi and InR ^CA ; mCherry ^RNAi data points were used as in Fig 6Q.
• F, G
  Muscle fillets stained with phalloidin (Actin) from tumour‐bearing (QRas ^V12 scrib ^RNAi ) animals where mCherry ^RNAi or sog was expressed in the fat body using r4‐GAL4.
• H
  Quantification of muscle detachment in (F, G). w ¹¹¹⁸ (n = 3), mCherry ^RNAi (n = 10), sog (n = 8).
• I, J
  Muscle fillets stained with phalloidin (Actin) from upon tumour‐specific overexpression of mCherry ^RNAi or Sog in Ras ^V12 dlg1 ^RNAi tumour‐bearing animals.
• K
  Quantification of muscle detachment in (I, J). w ¹¹¹⁸ (n = 3), mCherry ^RNAi (n = 4), sog (n = 6).
• L, M
  Muscle fillets stained with phalloidin (Actin) from tumour‐bearing animals with overexpression of luciferase or sog in the muscle via MEF2‐GAL4.
• N
  Quantification of muscle detachment in (L, M). w ¹¹¹⁸ (n = 3), luciferase (n = 10), Sog (n = 16).
• O, P
  Fat body stained with the ECM protein Nidogen from QRas ^V12 scrib ^RNAi tumour‐bearing animals, where mCherry ^RNAi or UAS‐sog was expressed in the fat body (r4‐GAL4).
• Q
  Quantification of normalised fat body Nidogen staining in (O, P). mCherry ^RNAi (n = 9), UAS‐sog (n = 10). Scale bar is 50 μm for fat body.
• R
  Sog levels in the haemolymph of w ¹¹¹⁸ , Ras ^V12 dlg1 ^RNAi and UAS‐TIMP; Ras ^V12 dlg1 ^RNAi tumour‐bearing animals (n = 4,4,3).

Data information: Scale bar is 200 μm for muscle fillet done at day 7 after tumour induction (F, G, I, J, L,M) and scale bar is 50 μm for fat body staining done at day 5 for wildtype fat body (A–D′) and 6 days after tumour induction (O, P). Graphs are represented as Mean ± SEM, n = the number of samples. (*) P < 0.05 (**) P < 0.01, (****) P < 0.0001, (ns) P > 0.05. For experiments with two genotypes, two‐tailed unpaired student's t‐tests were used to test for significant differences. The Welch's correction was applied in cases of unequal variances. For experiments with more than two genotypes, significant differences between specific genotypes were tested using a one‐way ANOVA and a subsequent Šidák post‐hoc test.

Source data are available online for this figure.