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. 2023 Nov 21;24(12):e57268. doi: 10.15252/embr.202357268

Figure 2. 1 month of IF does not affect NSC proliferation, quiescence/activation transitions nor maintenance.

Figure 2

  • A
    2‐month‐old GlastCreERT2;RYFP mice were administered tamoxifen on 5 consecutive days to fluorescently (YFP) label NSCs, after which, they were subjected to every‐other‐day IF for 1 month. EdU was administered in the drinking water for 5 days, 15 days before the analysis.
  • B, C
    Images of proliferating NSCs in control and IF mice, and quantification of the percentage of proliferating NSCs. NSCs were identified by their localisation in the SGZ, the presence of a single GFAP+ vertical projection and the help of YFP. Nuclear colocalisation with Ki67 was used to distinguish proliferating (Ki67+, arrowheads) from quiescent (Ki67−, arrow) NSCs. The Ki67− NSC is only shown for comparison, as it is unlikely to be the daughter cell of the proliferating NSC in the same picture. The percentage of proliferating NSCs was unchanged by IF. Two‐tailed unpaired t‐test, P = 0.9165.
  • D, E
    Images of EdU retaining NSCs in control and IF mice and quantification of their percentage. Arrowheads indicate EdU+ NSCs. IF did not affect the percentage of label retaining NSCs. Two‐tailed unpaired t‐test, P = 0.7046.
  • F
    Total number of NSCs normalised to DG length per 40‐μm‐thick section. The number of NSCs is unaffected by IF. Two‐tailed unpaired t‐test, P = 0.4452.

Data information: Bars and error bars represent average + s.d.; dots represent individual mice; n control = 17, n IF = 14. Significance summary: ns, P > 0.05. Scale bars: 20 μm.

Source data are available online for this figure.