Skip to main content
. 2023 Nov 21;24(12):e57268. doi: 10.15252/embr.202357268

Figure 3. 3 months of IF do not affect NSC proliferation nor maintenance.

Figure 3

  • A
    2‐month‐old GlastCreERT2;RYFP mice were administered tamoxifen on 5 consecutive days to fluorescently (YFP) label NSCs, after which they were subjected to every‐other‐day IF for 3 months.
  • B
    Images of NSCs identified by their localisation in the SGZ, the presence of a single GFAP+ vertical projection, and the help of YFP. Arrowheads show several NSCs.
  • C, D
    Quantification of the percentage of proliferating NSCs and the total number of NSCs in control and IF mice normalised to DG length per 40‐μm‐thick section. IF mice show similar levels of proliferating and total NSCs to control mice. Two‐tailed unpaired t‐tests; (C) P = 0.6317, (D) P = 0.1779.

Data information: Bars and error bars represent average + s.d.; dots represent individual mice; n control = 11, n IF = 12. Significance summary: ns, P > 0.05. Scale bar: 50 μm.

Source data are available online for this figure.