Skip to main content
. 2023 Nov 13;24(12):e56984. doi: 10.15252/embr.202356984

Figure EV1. Age‐related change of mRNA levels and promoter activities of XRCC4 and Lig4.

Figure EV1

  • A
    Correlation between the relative XRCC4 mRNA level (normalized to GAPDH mRNA level) and donor age. Error bars represent the s.e.m. values. T test was performed to assess the significance of the regression slope. n = 14 donors.
  • B–G
    Analysis of the XRCC4 mRNA level in the young (20–29 years old) and aged (60–69 years old) humans. Mann–Whitney U test was performed to determine the significance of the results. The data were extracted from GTEx database. Box and whisker plots indicate the median (central line), the 25th to the 75th percentiles (box), and the minimum to the maximum values (whiskers). Heart young, n = 37, old, n = 313; brain young, n = 70, old, n = 1,317; blood young, n = 85, old, n = 295; stomach young, n = 44, old, n = 79; small intestine young, n = 28, old, n = 42; ovary young, n = 22, old, n = 42.
  • H
    Schematic diagram of the firefly luciferase reporter driven by Lig4 promoter.
  • I
    Comparison of Lig4 promoter activity in cells derived from young and aged individuals. Each dot represents one donor. Error bars represent the s.e.m. values. n = 7 biological replicates. Mann–Whitney U test was performed to determine the significance of the results.
  • J
    Analysis of the correlation between the relative Lig4 promoter activity and donor age. Error bars represent the s.e.m. values. T test was performed to assess the significance of the regression slope in (G) and (H). All experiments were repeated for at least three times.