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. 2023 Nov 13;24(12):e57528. doi: 10.15252/embr.202357528

Figure EV1. RNF144A promotes DNA virus‐ or exogenous cytosolic DNA‐triggered innate immune responses.

Figure EV1

  1. HaCaT keratinocytes were transfected with the empty vector (Vec) or the RNF144A plasmid for 24 h and then treated with HSV60 (1 μg/ml), HT‐DNA (1 μg/ml), or left untreated for 8 h. The cells were lysed for real‐time PCR assays.
  2. PMA‐THP1 cells were transfected with control siRNA (SC) or RNF144A‐specific siRNA (A2). At 24 h after transfection, the cells were transfected with HSV60 (1 μg/ml), HT‐DNA (1 μg/ml), or ISD (1 μg/ml) for 8 h. The cells were lysed for real‐time PCR assays.
  3. PMA‐THP1 cells were transfected with control siRNA (SC) or RNF144A‐specific siRNA (A2, A3). At 24 h after transfection, the cells were transfected with poly(dA:dT) (1 μg/ml), HT‐DNA (1 μg/ml), or poly(I: C) (2.5 μg/ml) for 8 h. The cells were lysed for immunoblot assays.
  4. Wild‐type (WT) and Rnf144a‐deficient (KO) PMA‐THP1 cells were infected with HSV‐1 (MOI = 1) for 8 h. The cells were lysed for immunoblot assays.
  5. Wild‐type (WT) and Rnf144a‐deficient (KO) PMA‐THP1 cells were infected with HSV‐1 (MOI = 1) for 24 h. The supernatants were collected and subjected to ELISA assays.
  6. Wild‐type (WT) and Rnf144a‐deficient (KO) PMA‐THP1 cells were infected with HSV‐1 (MOI = 1) for the indicated periods. The cells were lysed for real‐time PCR assays.
  7. Wild‐type (WT) and Rnf144a‐deficient (KO) PMA‐THP1 cells were infected with HSV‐1 (MOI = 1) for the indicated periods. The immunoblot assays were then performed.

Data information: Two‐tailed unpaired Student's t‐test, *P < 0.05, **P < 0.01 and ***P < 0.001. Data shown are representative of at least three independent biological replicates. In (A, B, F), each data point represents a technical replicate. In (E), each data point represents an independent biological replicate. Error bars are presented as mean ± SD.

Source data are available online for this figure.