Whole cell IRE1α oligomerization states under galactose conditions assessed by blue‐native PAGE. BN‐PAGE of IRE1α with whole cell extracts does not capture doxycycline regulation of high‐order oligomers when compared to ER concentrated at the mitochondria (Mito/ER extracts, Fig
4B). Glimepiride (25 μM) and IRE1α inhibitor 4μ8C (20 μM), compounds previously identified to rescue complex I mutants from nutrient deprivation, were used as controls. Note compensatory increase in IRE1α expression and oligomerization in 4μ8C treated cells.