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. 2023 Nov 8;24(12):e56964. doi: 10.15252/embr.202356964

Figure EV1. Generating OTCs.

Figure EV1

  1. Schematic setup of generating 150‐μm‐thick mouse brain slices (OTC) and cutting them along the hemisphere into four intersections and placing them on a Millicell culture insert. Scheme created with BioRender.com.
  2. 3D rendering of the CAD design of the thermoforming positive molds for the LSFM holders. An array of eight positive molds is shown. The molds have a square 2 × 2 mm cross‐section and a height of 5 mm. On the right panel, the real 3D‐printed molds array is displayed.
  3. One thermoformed FEP‐foil LSFM holder (3 × 25 mm) glued to a sample holder, followed by an OTC is placed inside (right panel).
  4. Table depicting the mean and SEM of the tumor cell dispersion of one biological replicate (n = 2 tumors per region) of spheres placed onto different brain regions depicted in (A) using GraphPad Prism 9.