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. 2023 Oct 17;24(12):e56327. doi: 10.15252/embr.202256327

Figure 3. CNOT1/3 dependence is specific to HCMV and consistent between strains.

Figure 3

  1. Immunoblot analysis of lysates from NHDF cells transfected with the indicated siRNAs and infected with vaccinia virus (VACV) or Herpes Simplex Virus 1 (HSV‐1) at MOI = 0.005. Following multicycle infection, lysates were collected at 72 HPI and probed for representative HSV‐1 proteins from each temporal expression class (ICP4, US3, gC), for vaccinia proteins using a pan‐vaccinia antibody, and for tubulin as a loading control.
  2. Immunoblot analysis of lysates from NHDF cells transfected with the indicated siRNAs and infected with HCMV clinical strain TB40/E at MOI = 3, collected at 72 HPI. Lysates were probed for representative viral proteins from each temporal expression class and GAPDH.

Source data are available online for this figure.