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. 2023 Oct 17;24(12):e56327. doi: 10.15252/embr.202256327

Figure EV4. The impact and functional importance of host transcriptome changes upon CNOT1/3 knockdown.

Figure EV4

  1. NHDFs were pretreated with indicated cytokines for 24 h and subsequently infected with HCMV (AD169) at low MOI (0.05). At 7 DPI‐infected cells were identified as in Fig 1A using high content imaging. Mean % infected cells ± SEM, normalized to nontreated (NT) cells (n = 3 biological replicates) is plotted. Statistical significance established by ANOVA test with Dunnett multiple comparison correction compared control siRNA‐treated samples; (ns) P > 0.033, (***) P < 0.001.
  2. Select differentially regulated transcripts were validated by RT‐qPCR analysis from control or CNOT1 siRNA‐transfected cells that were mock infected or infected with HCMV AD169 at MOI = 3 and collected at 72 HPI. Mean ± SEM (n = 3 biological replicates) fold change relative to GAPDH is plotted.
  3. siRNAs against host genes differentially expressed by CNOT1/3 knockdown were validated by RT‐qPCR 3 days post‐transfection of NHDFs.

Data information: Statistical significance in (B) and (C) was tested by students t‐tests; (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, no asterisk: not significant.