TABLE 1.
Effects of divalent cations on His6-BCTD GTPase and ATPase activitiesa
| Cation | % Activity (mean ± SE; n = 6)
|
|
|---|---|---|
| GTPase | ATPase | |
| None | 0 | 0 |
| Mg2+ | 100 | 100 |
| Mn2+ | 165 ± 14.5 | 125 ± 9.8 |
| Ca2+ | 0 | 0 |
| Zn2+ | 0 | 0 |
| Co2+ | 15 ± 1.8 | 20 ± 2.8 |
GTPase and ATPase activities were measured with a nucleotide concentration of 10 μM as described in Materials and Methods; cations (5 mM) in their chloride form were used. Activities are standardized to that of the reaction mixture containing Mg2+.