TABLE 2.

Effects of ATPase inhibitors on His₆-BCTD GTPase and ATPase activities^a

Compound	% Activity (mean ± SE; n = 6)
	GTPase	ATPase	Inhibitor forb:
None	100	100
Vanadate (100 μM)	74 ± 8	90 ± 11	P
NaN3 (50 mM)	80 ± 7.6	90 ± 5.8	F
KNO3 (2 mM)	76 ± 12.3	84 ± 10.9	V
NEM (1 mM)	10 ± 2	18 ± 3.5

^aReaction mixtures contained the same protein and nucleotides as those for Table 1. His₆-BCTD was preincubated with the compounds for 10 min on ice before addition of nucleotides to initiate the reaction at 37°C. 

^bP, phosphorylation-type ATPase; F, F₀F₁-type ATPase; V, vacuolar-type ATPase.