Skip to main content
. 2023 Nov 21;24(12):e57269. doi: 10.15252/embr.202357269

Figure 2. 5:2 diet does not significantly affect cell proliferation in the dentate gyrus (DG).

Figure 2

  1. Representative light microscopy images of positive Ki67 immunoreactivity, indicative of proliferating cells, in the dentate gyrus (DG) of adolescent male mice. Scale bars represent 50 μm.
  2. Representative images of Ki67+ cells in adult male mice. Scale bars represent 50 μm.
  3. Average number of Ki67+ cells per DG section. Ki67 cell counts for each DG section were quantified using Image J. The effect of diet (ad libitum vs. 5:2 diet) was assessed in the presence and absence of functional ghrelin receptor (GHSR) expression (C57BL/6J vs. loxTB‐GHSR) for both male and female mice of both adolescent and adult age groups.

Data Information: Symbols represent individual mice, bars represent mean values, error bars represent SEM. Statistical comparisons made using ordinary 2‐way ANOVA followed by Šídák's multiple comparisons. ns ≥ 0.05. n = 5–8 mice per group.

Source data are available online for this figure.