Figure 5. 5:2 diet does not significantly affect the number of new (7‐week‐old) neural stem cells (NSCs) or astrocytes in the DG.

1. Representative maximum intensity projections (MIPs) of BrdU (488 nm), S100β (568 nm) and Sox2 (647 nm) immunofluorescence in the DG of adolescent and adult male C57BL/6J ad libitum fed mice. Images were acquired with an LSM980‐Airyscan2 confocal system (Zeiss), using the SR‐4Y airyscan mode. Scale bars represent 20 μm. Arrows indicate BrdU⁺/S100β⁺/Sox2⁺ cells.
2. Quantification of BrdU⁺/ Sox2⁺/S100β⁻ cells, indicative of new (7‐week‐old) neural stem cells, per DG section in adolescent and adult mice. Cells were manually counted. The effect of diet (ad libitum vs. 5:2 diet) was assessed in the presence and absence of functional ghrelin receptor (GHSR) expression (C57BL/6J vs. loxTB‐GHSR) for both males and females of both age groups.
3. Quantification of BrdU⁺/S100β⁺ cells, indicative of new astrocytes, per DG section in adolescent and adult mice (methodology same as B).
4. Quantification of BrdU⁺/ Sox2⁺/S100β⁻ cells in the rostral and caudal DG of adolescent mice. The effect of diet (ad libitum vs. 5:2 diet) was assessed independently for both sexes and both genotypes.
5. Quantification of BrdU⁺/ Sox2⁺/S100β⁻ cells in the rostral and caudal DG of adult mice (same methodology as D).

Data Information: Symbols represent individual mice, bars represent mean values, error bars represent SEM. Statistical comparisons made using ordinary 2‐way ANOVA for (B) and (C) and RM 2‐way ANOVA for (E) and (F). Šídák's multiple comparisons used for post hoc assessments. ns P ≥ 0.05. n = 5–8 mice per group.

Source data are available online for this figure.