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. 2023 Nov 21;24(12):e57269. doi: 10.15252/embr.202357269

Figure 6. 5:2 diet does not significantly affect object in place (OIP) or open field (OF) test performance.

Figure 6

  1. Schematic illustration of the OIP test.
  2. Comparison of OIP discrimination ratios, indicative of spatial discrimination performance. The effect of diet (ad libitum vs. 5:2 diet) was assessed in the presence and absence of functional ghrelin receptor (GHSR) expression (C57BL/6J vs. loxTB‐GHSR) for both males and females of both age groups.
  3. Schematic illustration of the OF test arena. Following a 30‐min habituation period, mice were placed into the centre of the 80 × 80 cm test arena and allowed to freely explore for 10 min.
  4. Representative heat maps of movement in the OF test arena for adolescent male mice. Heat maps generated from videos of the 10‐min exploratory task using Ethovision XT13 software.
  5. Comparison of overall distance (cm) travelled in the OF test arena during the 10‐min exploration (group analysis methodology same as B).
  6. Comparison of time (s) spent in the 40 × 40 cm inner zone of the OF test arena during the 10‐min exploration (group analysis methodology same as B).
  7. Comparison of inner zone entry frequency during the 10‐min exploration (group analysis methodology same as B).

Data Information: Symbols represent individual mice, bars represent mean values, error bars represent SEM. Statistical comparisons made using ordinary 2‐way ANOVA with Šídák's multiple comparisons used for post hoc assessments. ns P ≥ 0.05. n = 6–9 mice per group.

Source data are available online for this figure.