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. 2023 Nov 21;14:1288223. doi: 10.3389/fimmu.2023.1288223

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Copyright © 2023 Kong, Yang, Ding, Cheng and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immune response kinetics were analyzed from the perspective of trout ocular mucosa (OM) transcriptome after infection with F. columnare (n = 9 fish per group). (A) Volcano plots showing the DEGs distribution of OM in trout at 1 DPI (left) and 14 DPI (right) compared to controls. Red spots, expression fold change >2, FDR <0.05. Green spots, expression fold change <2, FDR <0.05. Black spots indicate no difference. The vertical axis represents-log10(FDR), and the horizontal axis represents log₂ (fold change). (B) Venn diagram of RNA-seq experiments showing overlap of up or downregulated genes in rainbow trout OM at 1 or 14 DPI compared to control fish.