FIG. 2.

Restriction map of pKM9372 and deletion analysis of pKM1. The directions of transcription by the lac promoter are indicated by arrowheads. The procedure for the measurement of LinD activity is described in Materials and Methods. The deduced location and direction of the linD gene is indicated by the arrow under the restriction map. The white bars above the map indicate the DNA fragments used as probes in Southern blot analyses. B, BamHI; E, EcoRI; H, HindIII; K, KpnI; M, MluI; N, NaeI; S, SacI.