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Southern blot analysis of UT26 and its Tn5-induced mutants probed with the linD gene. Lanes: 1, 5-kb EcoRI-HindIII fragment containing the linD gene; 2, λ DNA digested with HindIII; 3 to 6, total DNA of UT26, UT102, UT103, and UT116, respectively, digested with EcoRI and HindIII. The conditions of the experiment are described in Materials and Methods.
