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. 1998 Mar;180(6):1354–1359. doi: 10.1128/jb.180.6.1354-1359.1998

FIG. 5.

FIG. 5

TLC analysis of the 2,5-DCHQ degradation activity of E. coli overproducing the LinD protein (A) and E. coli overproducing the LinB protein (negative control) (B). Lanes: 1, authentic 2,5-DCHQ (spot 1); 2, authentic CHQ (spot 2); 3, authentic HQ (spot 3); 4, extraction from E. coli MV1190 cells overproducing LinD or LinB; 5 to 12, extraction from E. coli MV1190 cells overproducing LinD or LinB incubated with 50 ppm of 2,5-DCHQ for 0 min, 15 min, 60 min, 90 min, 3 h, 6 h, 12 h, and 24 h, respectively. The procedure for TLC analysis was described in Materials and Methods.