TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| S. paucimobilis strains | ||
| UT26 | Growth on γ-HCH; Nalr | 8 |
| UT102 | Nalr Kmr | 18 |
| UT103 | Nalr Kmr | 18 |
| UT116 | Nalr Kmr | 18 |
| P. putida strain | ||
| PpY101 | met Nalr | 11 |
| E. coli strains | ||
| HB101 | F−hsdS recA ara proA lacY galK rpsL xyl mtl supE | 12 |
| MV1190 | Δlac-proAB thi supE Δsrl-recA306::Tn10 F′ traD36 proAB lacIqZΔM15 | 17 |
| Plasmids | ||
| pAQN | pMB9 replicon; lacIqaqna Apr | 23 |
| pKM1 | pUC18 carrying 3-kb SacI-HindIII fragment; direction of linD is identical to that of lac promoter of pUC18 | This study |
| pKM1R | pUC18 carrying same fragment as pKM1 with opposite direction | This study |
| pKM2 | pUC18 carrying 1.2-kb EcoRI-MluI fragment of pKM170 | This study |
| pKM2R | pUC18 carrying same fragment as pKM2 with opposite direction | This study |
| pKM170 | pUC18 carrying 2.0-kb fragment of pKM1; deletion plasmid with deletion about 900 bp from 5′ end of fragment inserted in pKM1 | This study |
| pKM9372 | pUC18 carrying 8-kb EcoRI fragment of pMM9372 | This study |
| pKS13 | RK2 replicon; cos Mob+ Tcr | 11 |
| pKSM937 | pKS13 with 20 kb of UT26 DNA containing linD | This study |
| pMFY42 | RSF1010 replicon; Kmr Tcr | 4 |
| pMM9372 | pMFY42 carrying 8-kb EcoRI fragment of pKSM937 | This study |
| pMYLB1 | pAQN carrying linB in place of aqna | 16 |
| pMYLD2 | pAQN carrying linD in place of aqna | This study |
| pRK2013 | ColE1::RK2 Tra+ Kmr | 3 |
| pUC18 | pMB9 replicon of pKM9372; Apr | 26 |
a
aqn, aqualysin I gene of Thermus aquaticus.