KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| non-specific human IgA | Abcam | Abcam Cat#ab91025 |
| anti-β-actin antibody | Cell Signaling Technology | Cell Signaling Technology Cat# 5125, RRID:AB_1903890 |
| anti-Phospho-p44/42 MAPK(ERK1/2) antibody | Cell Signaling Technology | Cell Signaling Technology Cat# 4370, RRID:AB_2315112 |
| anti-p44/42 MAPK(ERK1/2) antibody | Cell Signaling Technology | Cell Signaling Technology Cat# 9102, RRID:AB_330744 |
| anti-RAS (G12D Mutant Specific) (D8H7) antibody | Cell Signaling Technology | Cell Signaling Technology Cat# 14429, RRID:AB_2728748 |
| anti-mCherry antibody | Rockland | Rockland Cat# 600-401-P16, RRID:AB_2614470 |
| Goat anti-Human IgG (H+L) Secondary Antibody, HRP | Thermo Fisher Scientifi | Thermo Fisher Scientific Cat# A18805, RRID:AB_2535582 |
| Goat anti-Human IgA Secondary Antibody, HRP | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A18781, RRID:AB_2535558 |
| anti-Human Ig Kappa Light Chain Antibody | R&D Systems | R&D Systems Cat#MAB10050 |
| anti-J Chain Antibody | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# MA1-80527, RRID:AB_934333 |
| anti-PIGR antibody | Abcam | Abcam, ab96196, RRID:AB_10677612 |
| InVivoMAb anti-mouse CD8β (Lyt 3.2) | Bio X Cell | Bio X Cell Cat# BE0223, RRID:AB_2687706 |
| InVivoPlus rat IgG1 isotype control (anti-HRP) | Bio X Cell | Bio X Cell Cat# BE0088, RRID:AB_1107775 |
| anti-Secretory Component antibody | Abcam | Abcam Cat# ab3924, RRID:AB_2261963 |
| anti-IgA antibody | Abcam | Abcam Cat# ab124716, RRID:AB_10976507 |
| anti-IgG antibody | Abcam | Abcam Cat# ab109489, RRID:AB_10863040 |
| Mouse anti-human CD3 antibody | ThemoFisher Scientific | Thermo Fisher Scientific;Cat# MA5-14524, Clone SP7; RRID: AB_10982026 |
| Mouse anti-human PCK antibody | DAKO | Agilent Cat# M3515, Clone AE1/AE3; RRID: AB_2132885 |
| anti-RAB8A antibody | Proteintech | Proteintech Cat# 55296-1-AP, RRID:AB_10858398 |
| anti-RAB5A antibody | Proteintech | Proteintech Cat# 11947-1-AP, RRID:AB_2269388 |
| anti-RAB11A antibody | Proteintech | Proteintech Cat# 20229-1-AP, RRID:AB_10666202 |
| anti-RAB7A antibody | Proteintech | Proteintech Cat# 55469-1-AP, RRID:AB_11182831 |
| Bacterial and Virus Strains | ||
| One Shot™ TOP10 Chemically Competent E.coli | ThermoFisher Scientific | Cat# C404010 |
| Biological Samples | ||
| Lung tumor tissue microarray (TMA) | US BioMax, Inc. | LC953 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| RPMI 1640 Medium | Gibco™ | Cat# 11875093 |
| Penicillin/Streptomycin | Lonza | Cat# 17602E |
| L-glutamine | Genesee Scientific | Cat# 25509 |
| Sodium pyruvate | ThermoFisher Scientific | Cat# 11360070 |
| 0.25% Trypsin-EDTA | Gibco™ | Cat# 25200056 |
| Dulbecco's Phosphate Buffered Saline 1X | VWR Life Science | Cat# 0201190500 |
| DMEM Medium | Thermo Fisher Scientific | Cat# 11965092 |
| POLYBRENE, 10MG/ML AQUEOUS SOLUTION | Thermo Fisher Scientific | Cat# AB01643-00001 |
| Lipofectamine™ 3000 Transfection reagent | Invitrogen | Cat# L3000015 |
| DAPI | Sigma | Cat# 8417 |
| RIPA Lysis and Extraction Buffer | Thermo Fisher Scientific | Cat#89900 |
| Protease/Phosphatase Inhibitor Cocktail (100X) | Cell Signaling | Cat#5872 |
| Thiazolyl Blue Tetrazolium Bromide, MTT | Millipore Sigma | Cat#M2128 |
| Hoechst 33342 Solution | Thermo Fisher Scientific | Cat#62249 |
| MRTX1133 | MedChemExpress | Cat#HY-134813 |
| Tris-Glycine Native Sample Buffer | Invitrogen | Cat#LC2673 |
| Novex™ WedgeWell™ 12% Tris-Glycine Gel | Invitrogen | Cat# XP00120BOX |
| Invitrogen™ Colloidal Blue Staining Kit | Invitrogen | Cat#LC6025 |
| ECL™ Prime Western Blotting System | Millipore Sigma | GERPN2232 |
| Dynabeads™ MyOne™ Streptavidin T1 | Invitrogen | Cat#65601 |
| InVivoPure pH 7.0 Dilution Buffer | BioXCell | Cat#IP0070 |
| Critical Commercial Assays | ||
| Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® | abcam | Cat#ab201796 |
| Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® | abcam | Cat#ab236553 |
| Pierce™ BCA Protein Assay Kit | Thermo Fisher Scientific | Cat#23225 |
| Pierce™ Co-Immunoprecipitation Kit | Thermo Fisher Scientific | Cat#26149 |
| APC Annexin V, 100 test | Biolegend | Cat#640920 |
| LigaTrap® Human IgG Purification Kit Product Instructions | LigaTrap Technologies | Cat#LT-095KIT |
| LigaTrap® Human IgA Purification Kit, 10 x 0.10 mL | LigaTrap Technologies | Cat#LT-146KIT |
| Deposited Data | ||
| Mass spectrometry proteomics data | This paper | PRIDE:XXX |
| Experimental Models: Cell Lines | ||
| OVCAR3 | ATCC | (RRID:CVCL_0465) |
| A427 | ATCC | (RRID:CVCL_1055) |
| SK-LU-1 | ATCC | (RRID:CVCL_0629) |
| HEK293T | ATCC | (RRID:CVCL_0063) |
| NCI-H23 | ATCC | (RRID:CVCL_1547) |
| NCI-H647 | ATCC | (RRID:CVCL_1574) |
| IDH1+/+ HCT116 | Horizon Discovery | (RRID:CVCL_0291) |
| IDH1R132H/+ HCT116 | Horizon Discovery | (RRID:CVCL_0291) |
| Brpkp110 | PMID: 27803104 | N/A |
| KPMSH2KIN | This manuscript | N/A |
| PIGR-ablated OVCAR3 | PMID: 33536615 | N/A |
| KRASG12D-PamCherryOVCAR3 | This manuscript | N/A |
| KRASwt-PamCherryOVCAR3 | This manuscript | N/A |
| Experimental Models: Organisms/Strains | ||
| NOD.129S7(B6)-Rag1tm1Mom/J | The Jackson Laboratory | RRID:IMSR_JAX:003729 |
| C57BL/6J mice | The Jackson Laboratory | RRID:IMSR_JAX:000664 |
| NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | The Jackson Laboratory | RRID:IMSR_JAX:005557 |
| Recombinant DNA | ||
| pLVX-IRES-Zs | GreenTakara Bio | Cat# 632187 |
| pLenti-TO/CMV-PAmCherry1-KRASWT | PMID: 26080442 | N/A |
| pLenti-TO/CMV-PAmCherry1-KRASG12D | PMID: 26080442 | N/A |
| pMD2.G | Addgene | Cat# 12259; RRID: Addgene_12259 |
| psPAX2 | Addgene | Cat# 12260; RRID: Addgene_12260 |
| J-chain encoding pcDNA3.0 vector | Addgene | RRID:Addgene_145146 |
| anti-IDH1R132HVH-IgA1 encoding PBMN-I-GFP | Genscript | N/A |
| anti-IDH1R132HVH-IgG4 encoding PBMN-I-GFP | Genscript | N/A |
| anti-IDH1R132HVL-Kappa encoding pVitro1 | Genscript | N/A |
| anti-KRASG12DVH-IgA1 encoding PBMN-I-GFP | Genscript | N/A |
| anti-KRASG12DVH-IgG4 encoding PBMN-I-GFP | Genscript | N/A |
| anti-KRASG12DVL-Kappa encoding pVitro1 | Genscript | N/A |
| Software and Algorithms | ||
| FlowJo v10.7.2 | FlowJoLLC | N/A |
| GraphPad Prism v9 | GraphPad Software Inc. | N/A |
| Adobe Photoshop 2022 | Adobe | N/A |
| Adobe Illustrator 2022 | Adobe | N/A |