FIG. 2.
Time course of the production of the cwlJ-lacZ fusion protein (A) and Northern blot analysis of the cwlJ region (B). (A) cwlJ-directed β-galactosidase activity of strain cbQ was determined at the indicated times after the onset of sporulation. Squares, cell growth at an optical density of 600 nm (OD600); diamonds, β-galactosidase activity. (B) Northern hybridization performed with the cwlJ-specific RNA probe as described in Materials and Methods. The lanes contained 5 μg of RNA from B. subtilis 168 at t−2 (−2), t0 (0), t2 (2), or t4 (4), and B. subtilis 1S86 (SigF−), 1S60 (SigE−), SpoIIIGΔ1 (SigG−), and 1S38 (SigK−) at t1.5 (1.5), t3 (3), t4.5 (4.5), or t6 (6). 0.47 indicates the size (in kilobases) of the hybridizing RNA in comparison with the migration of 23S and 16S RNAs.