TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| B. subtilis | ||
| 168 | trpC2 | D. Ehrlich |
| 1S38 | trpC2 spoIIIC94 | BGSCa |
| 1S60 | leuA8 tal-1 spoIIG41 | BGSC |
| 1S86 | trpC2 spoIIA1 | BGSC |
| SpoIIIGΔ1 | trpC2 spoIIIGΔ1 | 10 |
| cbQ | cwlJ::pMUTin2cJ | This study |
| 168SB | sleB::Spr | This study |
| CJSB | cwlJ::pMUTin2cJ sleB::Spr | This study |
| E. coli | ||
| JM109 | recA1 Δ(lac-proAB) endA1 gyrA96 thi-1 hsdR17 relA1 supE44 [F′ traD36 proA+B+ lacIqlacZΔM15] | Takara |
| C600 | supE44 hsdR thi-1 thr-1 leuB6 lacY1 tonA21 | Laboratory stock |
| Plasmids | ||
| pMUTin2 | lacZ lacI bla erm | D. Ehrlich |
| pGEM-3Zf(+) | lacZ bla | Promega |
| pMUTin2cJ | lacZ lacI ΔcwlJ bla erm | This study |
| pGEMcJ | lacZ bla ΔcwlJ | This study |
| pUCSO | lacZ AprsleB | This study |
| pDG1727 | Spr | BGSC |
| pUCSOSP | sleB::Spr | This study |
a
BGSC, Bacillus Genetic Stock Center, Ohio State University.