Fig 7. Inactivation of TGFß/Activin signaling diminished ecdysone biosynthesis.

(A) Dorsal and ventral view of a Control L7 larva and pupa respectively, and dorsal views of double knockout Tc-jhamt^RNAi and Tc-myo^RNAi, and Tc-jhamt^RNAi and Tc-babo^RNAi L7 larvae, 50 days after injection. Scale bar, 0.5 mm. (B, C) Transcript levels of Tc-phm, (B) and Tc-HR3 (C) measured by qRT-PCR in 2-day-old L7 Control, Tc-babo^RNAi and Tc-myo^RNAi larvae. Transcript abundance values were normalized against the Tc-Rpl32 transcript. Average values of three independent datasets are shown with standard errors (n = 6). Asterisks indicate statistically significant differences at **p < 0.01 and ***p < 0.001 (t-test). Raw data of B and C are in S1 Data (tab Fig 7).