. 2023 Dec 7;12:RP89795. doi: 10.7554/eLife.89795

Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Antibody	Anti-GFP (rabbit polyclonal)	Thermo Fisher Scientific	Cat# A6455, RRID:AB_221570	IF (1:1000)
Antibody	Anti-mCD8 (rat monoclonal)	Thermo Fisher Scientific	Cat# MCD0800, RRID:AB_10392843	IF (1:200)
Antibody	Anti-rabbit Alexa 488 (goat polyclonal)	Thermo Fisher Scientific	Cat# A11070, RRID:AB_2534114	IF (1:500)
Antibody	Anti-rat Cy3 (goat polyclonal)	Jackson ImmunoResearch Laboratories Inc	Cat# 112-165-072, RRID:AB_2338248	IF (1:300)
Chemical compound, drug	Caffeine	MilliporeSigma	C0750
Chemical compound, drug	Capsaicin	MilliporeSigma	M2028
Chemical compound, drug	Denatonium benzoate	MilliporeSigma	D5765
Chemical compound, drug	Lobeline hydrochloride	MilliporeSigma	141879
Chemical compound, drug	D-(-)-ribose	MilliporeSigma	R7500
Chemical compound, drug	Quinine hydrochloride dihydrate	MilliporeSigma	Q1125
Chemical compound, drug	Fructose	Spectrum Chemical	F1092
Chemical compound, drug	Agarose	Apexbio	20-102
Chemical compound, drug	Sucrose	Macron Fine Chemicals	8360-06
Chemical compound, drug	Charcoal	J.T. Baker	1560-01
Genetic reagent (Drosophila melanogaster)	w¹¹¹⁸	Bloomington Drosophila Stock Center	BDSC: 3605; FLYB: FBst0003605
Genetic reagent (D. melanogaster)	Gr28a-GAL4	Thorne and Amrein, 2008	FLYB: FBtp0056017	FlyBase symbol: w; P{Gr28a-GAL4.T}SF36S*
Genetic reagent (D. melanogaster)	Gr28a-GAL4	Thorne and Amrein, 2008	FLYB: FBtp0056017	FlyBase symbol: w; P{Gr28a-GAL4.T}SF36B1*
Genetic reagent (D. melanogaster)	Gr28b.a-GAL4	Thorne and Amrein, 2008	FLYB: FBtp0054526	FlyBase symbol: w; P{Gr28b.a-GAL4}NT42aC51a*
Genetic reagent (D. melanogaster)	Gr28b.c-GAL4	Thorne and Amrein, 2008	FLYB: FBtp0054528	FlyBase symbol: w; P{Gr28b.c-GAL4}NT21B1*
Genetic reagent (D. melanogaster)	Gr28b.e-GAL4	Scott et al., 2001	FLYB: FBtp0014672	FlyBase symbol: w; P{Gr28b.e-GAL4.4.245}Gr28a3AII*
Genetic reagent (D. melanogaster)	ΔGr28/ΔGr28	Mishra et al., 2018	FLYB: FBab0049019	FlyBase symbol: w; Df(2L)ΔGr28*
Genetic reagent (D. melanogaster)	Gr66a-GAL4	Scott et al., 2001	FLYB: FBtp0014661	FlyBase symbol: w; P{Gr66C1-GAL4.3.153}*
Genetic reagent (D. melanogaster)	UAS-Gr28a	Ni et al., 2013	FLYB: FBal0344045	FlyBase symbol: w; P{UAS-Gr28a.G}attP2*
Genetic reagent (D. melanogaster)	UAS-Gr28b.a	Ni et al., 2013	FLYB: FBal0291410	FlyBase symbol: w; P{UAS-Gr28b.A}attP2*
Genetic reagent (D. melanogaster)	UAS-Gr28b.b	Ni et al., 2013	FLYB: FBal0291412	FlyBase symbol: w; P{UAS-Gr28b.B}attP2*
Genetic reagent (D. melanogaster)	UAS-Gr28b.c	Ni et al., 2013	FLYB: FBal0291411	FlyBase symbol: w; P{UAS-Gr28b.C}attP2*
Genetic reagent (D. melanogaster)	UAS-Gr28b.d	Ni et al., 2013	FLYB: FBal0291409	FlyBase symbol: w; P{UAS-Gr28b.D}attP2*
Genetic reagent (D. melanogaster)	UAS-Gr28b.e	Ni et al., 2013	FLYB: FBal0291408	FlyBase symbol: w; P{UAS-Gr28b.E}attP2*
Genetic reagent (D. melanogaster)	Gr43a^GAL4	Miyamoto et al., 2012	BDSC:93447; FLYB:FBst0093447	FlyBase symbol: w¹¹¹⁸;Ti{GAL4}Gr43a^GAL4
Genetic reagent (D. melanogaster)	UAS-VR1E600K	Marella et al., 2006	FLYB: FBal0215202	FlyBase symbol: w¹¹¹⁸;P{UAS-VR1E600K}
Genetic reagent (D. melanogaster)	lexAop-rCD2:GFP	Lai and Lee, 2006	FLYB: FBst0066687	FlyBase symbol: w; P{lexAop-rCD2-GFP}*
Genetic reagent (D. melanogaster)	UAS-mCD8:RFP	Bloomington Drosophila Stock Center	BDSC: 32220; FLYB: FBti0131987	FlyBase symbol: y¹w;P{10XUAS-IVS-mCD8::RFP}su(Hw)attP8*
Genetic reagent (D. melanogaster)	UAS-GCaMP6m	Bloomington Drosophila Stock Center	BDSC: 42748; FLYB: FBti0151346	FlyBase symbol: w¹¹¹⁸; P{20XUAS-IVS-GCaMP6m}attP40
Genetic reagent (D. melanogaster)	UAS-Kir2.1-GFP	Baines et al., 2001; Paradis et al., 2001	FLYB: FBst0006596	FlyBase symbol: w; P{UAS-Hsap\KCNJ2.EGFP}1*
Genetic reagent (D. melanogaster)	Gr66a-LexA	Thistle et al., 2012	BDSC: 93024; FLYB: FBst0093024	FlyBase symbol: w¹¹¹⁸; P{Gr66a-lexA.S}2;TM2/TM6B
Genetic reagent (D. melanogaster)	lexAop-GAL80	Thistle et al., 2012	FLYB: FBtp0079728	FlyBase symbol: w¹¹¹⁸; P{lexAop-GAL80. T}
Genetic reagent (D. melanogaster)	Gr28b.c-LexA	This paper		FlyBase symbol: w¹¹¹⁸;P{Gr28b.c-LexA}#8
Sequence-based reagent	Gr28b.c_F	This paper	PCR primers	5′-AATCTAGGTACCCCGGCTGCTCGTCTCCCTGGATGT-3′
Sequence-based reagent	Gr28b.c_R	This paper	PCR primers	5′-CGTCAAACTAGTGACCGCTTCGTTTGAGCTTCAACC-3′
Recombinant DNA reagent	LexA vector CMC105 (plasmid)	This paper Larsson et al., 2004		Insect expression vector
Software, algorithm	NIS-Elements	Nikon	N/A
Software, algorithm	Prism software 10.1.0 (264)	GraphPad Software	N/A
Software, algorithm	Adobe pPhotoshop 2022	Adobe	N/A
Other	Normal goat serum	SouthernBiotech	Cat# 0060-01	IF (5%) ‘Materials and methods’
Other	Nikon Eclipse Ti inverted microscope	Nikon	N/A	‘Materials and methods’
Other	Nikon A1R confocal microscope system	Nikon	N/A	‘Materials and methods’
Other	PertriPetri dish, 60 × 15 mm	Falcon	REF353004	‘Materials and methods’
Other	Microscope cover glass, 24 × 50 mm	VWR	16004-098	‘Materials and methods’
Other	Microscope cover glass, 12CIR-1	Thermo Fisher Scientific	1254580	‘Materials and methods’