Table 3.
Statistics on the editing efficiency of EcIAG mediated by CRISPR/Cas9 system.
| Target gene | Injection | Editing efficiency | Hatching rate | Survival adult | Adult mutant | |||
|---|---|---|---|---|---|---|---|---|
| EcIAG | Injected at zygote stage | Injected at the first cleavage | Injected at zygote stage | Injected at the first cleavage | (40/266) 15.04% |
20 | heterozygous mutant | homozygous mutant |
| 400 | 200 | 22.48% | 13.95% | 5 | 3 | |||