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Effect of a SecA inhibitor on precursor processing of the mutants. Cells were treated with sodium azide for 1 min prior to being labeled with [35S]methionine for 40 s. (A) Autoradiogram of the gel pattern. The positions of the precursor and mature forms of alkaline phosphatase are indicated by p and m, respectively. (B) The percentages of total alkaline phosphatase observed in the precursor form with and without azide are summarized.
