TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant phenotype(s) or genotype(s) | Source or reference |
|---|---|---|
| Strains | ||
| S. thermophilus | ||
| CNRZ368 | Wild-type strain | INRA-CNRZ, strain collection |
| 16D10 | EmrS. thermophilus CNRZ368 containing integrated pGh9:ISSI into cse | This work |
| CNRZ368-Δcse | S. thermophilus CNRZ368 with Δcse mutation | This work |
| CNRZ368-Δcse-T | Emr, CNRZ368-Δcse with pNST260+ integrated into the chromosome | This work |
| CNRZ368-Δcse-C | Emr, CNRZ368-Δcse with pNST260+::cse integrated into the chromosome | This work |
| LMG18311 | Wild-type strain | BCCM/LMG, strain collection |
| LMG18311-Δcse | S. thermophilus LMG18311 with Δcse mutation | This work |
| LMG18311-csevarCNRZ368 | S. thermophilus LMG18311 with the chimeric allele cseLMG18311varCNRZ368 | This work |
| L. lactis subsp. cremoris MG1363acmAΔ1 | Derivative of MG1363 wild-type strain containing 701-bp SacI-SpeI chromosomal deletion in acmA gene | 6 |
| E. coli | ||
| EC101 | supE hsd-5 thi Δ(lac-proAB) F′ (traD6 proAB+lacIqlacZΔM15) repA+, derivative of TG1 strain (47) | 23 |
| DH5α | supE44 ΔlacU169 (φ80 lacZ ΔM15) hsdR17 endA1 gyrA96 thi-1 relA1 | 47 |
| BL21 (DE3) | hsdS gal (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene I) | 47 |
| Plasmids | ||
| pGh9 | Emr, thermosensitive replication origin from pVE6002 | 27 |
| pGh9:ISS1 | pGh9 with ISS1 | 28 |
| pGh9::ΔcseCNRZ368 | pGh9 with Δcse and CNRZ368 surrounding regions | This work |
| pGh9::ΔcseLMG18311 | pGh9 with Δcse and LMG18311 surrounding regions | This work |
| pGh9::cseLMG18311varCNRZ368 | pGh9 with chimeric allele cseLMG18311varCNRZ368 | This work |
| pNST260+ | pGh9 with int and attI from ICEStI | G. Guédon, personal communication |
| pNST260+::cse | pNST260+ with CNRZ368 cse ORF, its putative promoter and terminator | This work |
| pFUN | Emr and Ampr, derivative of pIL252, with ΔSPnuc gene | 38 |
| pFUN::cse | pFUN with cse-ΔSPnuc translational fusion | This work |
| pET15b | Protein expression vector | Novagen |
| pET15b::cse | pET15b with hexa-His-Cse translational fusion | This work |
| pET15b::cse1-183 | pET15b with hexa-His-Cse1-183 translational fusion | This work |
| pET15b::cse184-461 | pET15b with hexa-His-Cse184-461 translational fusion | This work |