TABLE 2.
Oligonucleotides used in this work
| Oligonucleotide | Sequencea (5′-3′) |
|---|---|
| delcse.E5′ | CCCCCCCCAAGCTTACAAGATCAGTTTGGAAC |
| delcse.I5′ | CCCCCCGAATTCTGATAACATGTATAATTCAT |
| delcse.I3′ | CCCCCCGAATTCATTTATCCATAATGAGTACA |
| delcse.E3′ | CCCCCCCTGCAGCAGCCTGTGGTTGAAG |
| fcompl | TTGTTGGCGCGCTTTAGCCGATTTGGCTTTTGAAG |
| rcompl | TTGTTGGCGCGCCCCATTATTTTCTCAGGATGAAT |
| racse.E5′ | CCCCCCAAGCTTTGAATGTTTTGGCTAATATC |
| racse.I5′ | CCCCCCGATATCAATAGCGTCTGCAACT |
| racse.I3′ | CCCCCCGATATCAACTCCTAATACATATC |
| racse.E3′ | CCCCCTGCAGTTATGGATAAATATAATATAC |
| ravar.5′ | CATGTTACTTCAGCAACAAC |
| ravar.3′ | GCATATTCGTATGTAGCTGC |
| fnuc.cse.5′ | CCCCCCGAATTCTGATAGGGATAGGGCTT |
| fnuc.cse.15 | CCCCCCAAGCTTCATGTATAATTCATTCCTT |
| fnuc.cse.13′ | CCCCCCAAGCTTGATGAAACTTCTCACTG |
| fnuc.cse.3′ | CCCCCCGGATCCCCTGGATAAATATAATATACAG |
| sex.E5′ | CCCCCCCCATATGTTATCAAAATCTAAAAC |
| sex.I5′ | CCCCGGATCCTTAAGCTGGAGTATCTGATGC |
| sex.I3′ | CCCCCCCCATATGTACGCAGATACTGAACAA |
| sex.E3′ | CCCCGGATCCTTATGGATAAATATAATATAC |
a
Restriction enzyme sites are underlined.