FIG. 4.

Identification of the BmpA, BmpB, and BmpD proteins and estimation of the relative expression of BmpA and BmpB in spirochetes cultured at 34°C. (A) Western blot showing the banding pattern of BmpA, BmpB, and BmpD. Three separate lanes from the same Western blot were developed with different anti-Bmp antibodies as follows: anti-BmpA monoclonal antibody (lane 1), polyclonal rhesus anti-BmpD antibody (lane 2), and mouse anti-BmpD polyclonal antibody (lane 3). The criterion used to deduce that the slowest-migrating band in lane 2 is BmpB is described in the text. (B) Western blot showing the expression of three Bmp proteins in four different 34°C cultures of B. burgdorferi JD1. The relative expression of the BmpA and BmpB proteins was quantified by densitometry.