FIG. 4.

Complementation of JS964 by a single-copy plasmid. pSEB12(minCDE) and derivatives of this plasmid containing minC mutations were transformed into JS964. Single colonies were inoculated into liquid cultures and grown at 37°C until the mid-log phase. Cell morphology was examined by phase-contrast microscopy, and pictures were taken with a MagnaFire charge-coupled device camera. (A) JS964; (B) JS964/pSEB12; (C) JS964/pSEB12 minC-R133A; (D) JS964/pSEB12 minC-E156A; (E) JS964/pSEB12 minC-R170A; (F) JS964/pSEB12 minC-R172A.