TABLE 2.
Effects of minC mutations on MinD and DicB interactions
| MinC mutant | Phenotypic assaya
|
Yeast two-hybrid assayb
|
||
|---|---|---|---|---|
| MinD activation (pZH106/pZH110) | DicB activation (pZH118/pZH110) | MinC-MinD | MinC-DicB | |
| R133A | − | +++ | − (0) | +++ (49) |
| S134A | + | +++ | +/− | +++ |
| D144A | +++ | +++ | +++ | +++ |
| E210A | +++ | +++ | +++ | +++ |
| E156A | + | − | + (1) | − (0) |
| R170A | ++ | +++ | +++ (69) | + (1) |
| R172A | + | + | ++ (35) | + (6) |
| D180A | − | − | − | − |
| E193A | +++ | +++ | ND | ND |
| D205A | +++ | +++ | ND | ND |
| Wild type | +++ | +++ | +++ (63) | +++ (48) |
a
+++, lethal; ++, severe filamentation; +, slight filamentation; −, no effect.
b
+++, dark blue developed in 30 min; ++, dark blue developed in 1 to 2 h;+, blue developed in 3 to 4 h; +/−, pale blue developed overnight; −, no color change. The numbers in parentheses are Miller units determined from the liquid assay. ND, not determined.