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. 2023 Dec 6;20(12):1989–1999. doi: 10.1038/s41592-023-02084-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a) Mitotic softening and stiffening in the 16AS, 24, 32AS, 64 and 120 cell stages of the early ascidian embryo (P. mammillata). Upper row: sagittal view of inferred apical tension. Middle and bottow rows: animal and vegetal views of the inferred cell pressures. The ratio of mitotic to interphase apical tension is colored green if it is less than 1 and red if it is greater than 1. The orientation of the embryo is given by arrows Ant: anterior, Pos: posterior, Med: medial, Lat: lateral, V: vegetal, A: animal. b) Vegetal view of stable (green) and unstable (red) junctions (Left) and tension inference 512 and 702 cell stages. The orientation of the embryo is given by arrows Ant: anterior, Pos: posterior, Med: medial, Lat: lateral.