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. 2023 Dec 6;20(12):1989–1999. doi: 10.1038/s41592-023-02084-7

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, The top shows the pressure inference on a simulated embryo confined in a rigid shell leading to heterogeneous mean curvatures patterns at cell–medium interfaces. The bottom shows a comparison of exact and inferred pressures (Laplace and variational Laplace formula). b, Inference of tension only on the C. elegans embryo at 4, 8 and 15 cell stages from microscopy images (source ref. ¹⁸). Scale bar, 10 μm.