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. 2023 Dec 5;20(12):2034–2047. doi: 10.1038/s41592-023-02080-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a, Schematic for the generation of MISCOs. b-c, Tilted side view and top view of PDMS embedding molds for linear fusions. d-e, size considerations for the fusion of three organoids. f-g, Side view of the PDMS embedding mold for MISCO generation. h, Cortical (TBR1⁺) and striatal (DARPP32⁺) tissue were innervated by dopaminergic axons (representative images, similar results in n = 8 MISCOs of 2 batches). i, Dopaminergic (TH⁺) neurons in the VM (white arrow) as well as their axons in striatum and cortex (yellow arrows) of day 90 MISCOs express the mature mDA marker Dopamine Transporter (DAT) on day 90 (representative images, similar results in n = 9/9 MISCOs). j, DARPP32⁺ neurons in 120-day-old MISCOs in the striatum expressed the striatal subtype markers DRD1 (yellow arrows) and DRD2 (white arrows) (representative images, similar results in n = 6-8 MISCOs of 2-3 batches). k-l, Dopaminergic neurons in MISCOs expressed the dopaminergic subtype markers GIRK2, CALB1, ALDH1A1, OTX2, GABA and SOX6 (n = 6|7|5|5|6|8 MISCOs of 3-4 batches). Yellow arrows: representative double-positive neurons. Data shown as mean ± SD.

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