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. 2023 Nov 13;20(12):1949–1956. doi: 10.1038/s41592-023-02057-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a U2OS cells expressing endogenously tagged paxillin were plated on 9.6 kPa polyacrylamide (PAM) gels and were imaged live using a spinning-disk confocal (SDC, resolution estimate: FRC = 573.0 ± 7.2 nm) and processed with eSRRF (resolution estimate: FRC = 197 ± 34 nm). b DCIS.com lifeact-RFP cells forming a spheroid in 3D matrigel in the presence of fluorescently labeled collagen I. Samples were fixed and imaged using a spinning-disk confocal microscope (resolution estimate: FRC(Actin)= 569 ± 59 nm/FRC(Collagen I) = 583 ± 14 nm) and processed using eSRRF (resolution estimate: FRC(Actin)= 229 ± 97 nm/FRC(Collagen I) = 130 ± 36 nm). Representative fields of view highlighting the spheroids’ middle and bottom planes are displayed. c Zebrafish embryos expressing mcherryCAAX in the endothelium were imaged live using a spinning-disk confocal. Vessels located at different parts of the embryo were imaged. For all panels, the eSRRF reconstructed images (resolution estimate: FRC(top) = 194 ± 37 nm/FRC(middle) = 393 ± 23 nm/FRC(bottom)= 307 ± 60 nm) and the original spinning disk images are displayed (resolution estimate: FRC(top) = 641 ± 82 nm/FRC(middle)= 573.9 ± 5.7 nm/FRC(bottom)= 575.9 ± 7.9 nm). The ROI zoom-ins are marked by white squares and the line profile position is marked in the eSRRF zoom-in by a white line. Intensity profiles of the diffraction-limited SDC data (dashed line) and eSRRF reconstruction are plotted in the panel on the very right. Scale bars 25 μm.