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. 2023 Nov 6;20(12):1900–1908. doi: 10.1038/s41592-023-02053-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — HeLa cells with GEM2/adaptor AAVS1 knock-in were cultured 48 h after transfection of EGFP-G3BP1 plasmid. GEM2 and adaptor expression from the AAVS1 locus was induced by 24 h doxycycline treatment. Cells were treated with rapalog for the indicated times under non-stress conditions. Formation of GEM2-EGFP-G3BP1 clusters in the cytoplasm are apparent at 4 h. Two experiments were performed with similar results.