Extended Data Fig. 10. Nanobody-free GEM2 labelling system.
a, Schematic of the system. b, HeLa cells with doxycycline-inducible GEM2-Halo-FKBP and Mito-mCherry-FRB AAVS1 knock-in were treated with rapalog for the indicated times. c, Analysis of b. Lines indicate mean, n = 41, 40, 42, 40 cells per treatment (left to right), 2 experiments. ***P < 0.0001, Kruskall-Wallis test followed by Dunn’s test, compared to 0 h rapalog treatment.