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. 2023 Nov 6;20(12):1900–1908. doi: 10.1038/s41592-023-02053-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — GFP-tagged proteins were overexpressed in HeLa cells with a GEM2/adaptor AAVS1 knock-in. Cells were cultured for 48 h after transfection of GFP-tagged protein plasmids. Rapalog treatment times are indicated for each target. For G3BP1, GEM2 labeling was induced before or after induction of stress granule formation via arsenite treatment. These are representative images, and the experiment was performed twice with similar results.