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. 2005 Apr;187(8):2618–2627. doi: 10.1128/JB.187.8.2618-2627.2005

FIG. 3.

FIG. 3.

Binding of E. coli FNR to the hyn regulatory region. (A) End-labeled hyn regulatory region fragment (carrying the sequence from position −113 to position 10) incubated with different concentrations of purified Ala154 FNR. The concentrations of FNR in the reaction mixtures were as follows: lane 1, no protein; lanes 2 to 7, 0.125, 0.25, 0.5, 1, 2, and 3 μM, respectively. (B) End-labeled hyn regulatory region fragments from pHYNP3 (FNRI+/FNRII+), pHYNP5 (FNRI+/FNRII−), and pHYNP6 (FNRI−/FNRII+) incubated with different concentrations of purified FNR. DNA was incubated with no protein (lanes 1, 5, and 9), 0.3 μM FNR (lanes 2, 6, and 10), 1 μM FNR (lanes 3, 7, and 11), and 3 μM FNR (lanes 4, 8, and 12).