FIG. 6.

Potassium permanganate footprint analysis of complexes formed in the hyn promoter region: cleavage products produced when the end-labeled pHYNP3 AatII-HindIII fragment was incubated with purified RNAP and FNR and then subjected to potassium permanganate footprint analysis. Lane 2 contained no protein. Lanes 3 to 5 contained 50 nM RNAP polymerase and the following concentrations of FNR: lane 3, no FNR; lane 4, 250 nM; lane 5, 500 nM. The gel was calibrated with a Maxam-Gilbert G+A sequencing reaction for the labeled fragment (lane 1). The locations of the permanganate-induced cleavage sites are indicated on the right.