TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Thiocapsa roseopersicina strains | ||
| BBS | Wild type | 7 |
| FNRTM | fnrT::Emr | This study |
| Escherichia coli strains | ||
| XL1-Blue MRF′ | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]c | Stratagene |
| M182 | E. coli K-12 Δlac | 11 |
| M182fnr | Like M182 but fnr | 6 |
| MC4100 | F−araD139 Δ(argF-lac)U169 ptsF25 deoC1 relA1 flbB350 rpsL150 λ− | 12 |
| RM313 | Like MC4100 but arcA1 zjj::Tn10 | 37 |
| RM315 | Like MC4100 but arcA1 zjj::Tn10, Δfnr | 37 |
| S17-1 (λpir) | 294 (recA pro res mod) Tpr Smr (pRP4-2-Tc::Mu-Km::Tn7), λ pir | 18 |
| Plasmids | ||
| pBluescript SK(+) | Ampr, cloning vector | Stratagene |
| pTSH2/8 | 4,631-bp BamHI fragment of the hyn operon in pBluescribe19+ | 30 |
| pGEM T-Easy | Ampr, cloning vector | Promega |
| pFNR1 | 262-bp fragment of fnrT of T. roseopersicina in pGEM T-Easy | This study |
| pFNR7 | 1,906-bp BamHI fragment of fnrT of T. roseopersicina in pBluescript SK(+) | This study |
| pPR9TT | RK2 vector with the promoterless lacZ gene, Ampr Cmr | 34 |
| pBBRMCS5 | Gmr, broad-host-range vector | 22 |
| pFLAC | Broad-host-range ′lacZ vector, Gmr | This study |
| pHYDPRO1 | 1,214-bp hynS promoter region in pBluescript SK(+) | This study |
| pHYDR1 | BamHI-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR2 | trhydo11-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR3 | trhydo12-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR4 | SphI-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR5 | PstI-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR6 | FspI-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR7 | Eco47III-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR8 | EcoRI-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR9 | hynpo12-HindIII fragment of pHYDPRO1 in pFLAC | This study |
| pHYDR17 | pHYDR9, but FNR site II mutated | This study |
| pHYDR18 | pHYDR9, but FNR site I mutated | This study |
| pK18mobsacB | Kmr, mob+sacB+ | 39 |
| pRL271 | Cloning vector carrying sacB, Emr (ermC) Cmr | GenBank accession no. L05081 |
| pFNRTM1 | SmaI-BamHI fragment of pFNR7 in SmaI-BamHI-digested pK18mobsacB | This study |
| pFNRTM2 | SalI-EcoRI fragment of pRL271 cloned in XhoI site of pFNRTM1 | This study |
| pSR | Ampr, pBR322 derivative for cloning EcoRI-HindIII promoter fragments, with an oop terminator sequence downstream of the HindIII site; also encodes a control RNA I | 20 |
| pHYNP1 | 132-bp hynpo7-hynpo8 PCR fragment in pBluescript SK(+) | This study |
| pHYNP3 | 148-bp EcoRI-HindIII fragment of pHYNP1 in pSR | This study |
| pHYNP5 | pHYNP3 but FNR site II mutated | This study |
| pHYNP6 | pHYNP3 but FNR site I mutated | This study |