Fig. 5.

Inhibition of Lox decreased collagen fibril production in DM hearts. (A) Representative immunofluorescence staining image displaying expression of Lox (red) stained with Col Ⅰ (green, fibroblast marker) and phalloidin (grey); scale bar = 10 µm. (B) Measurement of Lox activity of heart homogenates (n = 8, **p < 0.01). (C-D) Quantification of hydroxyproline (HYP) in heart tissues (n = 5) and serum (n = 8) (*p < 0.05, **p < 0.01). (E) Protein levels of Col Ⅰ and III by Western blotting. (F-G) Quantification of Col Ⅰ and III expression (n = 3, *p < 0.05, **p < 0.01). (H-K) Representative Masson and Sirius red staining images. In the Masson staining (H), the collagen area is blue, and the collagen area is red in the Sirius red staining (J). Quantification of results from Masson (I) and Sirius red staining (K) (n = 9, **p < 0.01). (L-P) Scatterplots showing correlation of Lox expression with HYP content, content of Col Ⅰ, content of Col III, and fibrosis area from the Masson and Sirius red staining. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)