Fig. 6.

Inhibition of Lox declined HG-induced collagen protein expression in primary fibroblasts.(A) Workflow of primary fibroblast culture. (B) Immunofluorescence staining showing the expression of Lox protein (Red), stained with Vimentin (green) and DAPI (blue); scale bar = 10 µm. (C) Western blot image showing the expression of Lox in fibroblasts. (D) Statistical analysis of Western blot results for Lox protein expression (n = 5, **p < 0.01). (E) Measurement of Lox activity in fibroblasts (n = 8, **p < 0.01). (F) Western blot image showing the expression of Col Ⅰ and III in the fibroblasts. (G-H) Quantification analysis of the expression of Col Ⅰ and III in fibroblasts (n = 5, *p < 0.05, **p < 0.01). (I-J) Scatterplots showing the correlation of Lox expression with Col Ⅰ or Col III protein content. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)