Table 1.
PacBio ISOseq output statistics.
| Samples | LL | LS | HL | HS | Average | |
|---|---|---|---|---|---|---|
| Summary of CCSa | ZMWb reads number | 1,458,258 | 1,151,319 | 1,436,305 | 1,191,697 | 1,309,395 |
| CCS reads number | 1,089,235 | 858,242 | 1,065,366 | 889,934 | 975,694 | |
| Read bases of CCS (bp) | 2,953,712,234 | 2,404,447,142 | 2,973,206,987 | 2,412,441,622 | 2,685,951,996 | |
| Mean Read length of CCS (bp) | 2,712 | 2,802 | 2,791 | 2,711 | 2,754 | |
| Summary of FLNCc | FLNC reads number | 1,002,196 | 804,915 | 1,012,454 | 837,039 | 914,151 |
| Read bases of FLNC (bp) | 2,641,851,236 | 2,178,718,162 | 2,739,795,345 | 2,177,595,795 | 2,434,490,135 | |
| Mean read length of FLNC (bp) | 2,636 | 2,707 | 2,706 | 2,602 | 2,663 | |
| GC content of FLNC (%) | 50.13% | 49.57% | 50.36% | 50.00% | 50.02% | |
| N50 of FLNC | 2,922 | 2,869 | 3,034 | 2,739 | 2,891 | |
Circular consensus sequencing (CCS) reads.
Zero-mode waveguide (ZMW) holes.
Full-length non-chimeric (FLNC) reads.