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Evaluation of species recoverability and species discrimination is depicted, comparing the efficacy of single barcodes, (rbcL, ITS2, trnH-psbA) and multigene-tiered barcodes, (rbcL + ITS2 + trnH-psbA, rbcL + ITS2 and rbcL + trnH-psbA) through similarity BLAST. Species recoverability is represented by the percentage of successfully sequenced species within each barcode, while species discrimination assesses the capability of the barcodes to achieve identification at the species level
