FIG. 2.

Effect of nickel and cobalt on growth. Overnight cultures of MC4100 (wt) (squares), ARY023 (yohM::uidA) (circles), and ARY023/pAR020 (yohM::uidA/yohM⁺) (triangles) in minimal medium supplemented with 0.4% glucose were diluted into 1:100 fresh identical medium with the indicated concentrations of NiCl₂ (A) or CoCl₂ (B). Cell growth was monitored as the OD₆₀₀ after 12 h of incubation at 37°C, with shaking, and converted to BDW. Experiments were performed in triplicate, and the values given are averages.