FIG. 3.
RT-PCR quantitation data indicating cAMP-mediated transcriptional regulation of differentially expressed proteins identified from 2-D GE studies. BCG cultures were grown with (solid bars) or without (hatched bars) exogenous cAMP (10 mM) shaking in 1.3% O2, 5% CO2 (1.3/5) or 20% O2, 5% CO2 (20/5). Genes examined included Rv1265, Rv2971, fixB, GroEL3, mdh, and PE_PGRS6a. Controls included tuf and normalized 16S RNA.