TABLE 1.
Primers used for RT-PCRb
| Gene | Primer sequencea |
|---|---|
| Rv1265c | F, ATG TAA TCA TGT AAT TAT GAG GC |
| R, GCG ATA GTC ATT CGC TCCG | |
| fixB | F, CGA CAA ATA CCT GAT CAC CC |
| R, GAA GAT GCT GTG GAC ACC C | |
| GroEL2 3′ | F, CAT CGC CGG ACG AGT GGC |
| R, CGA TCT GCT TCA GCG GGG | |
| GroEL2 5′ | F, GTA AAG GTG ACA TTG GGC |
| R, CGT TTG AGA CCG AGC G | |
| PPE39- | F, CAC CGG ATT TTG GAA CGG CG |
| R, GCG TGC CCG GAT TAA ACC C | |
| tuf | F, GTG CGG AAG TAG AAC TGC GG |
| R, AGG AAG TTG AGA TCG TCG GC | |
| Rv2971 | F, GCG CAA TTG CAG CCT CCG |
| R, TTG TCC AGC CTG CC | |
| mdh | F, GGA TGA CGT TCG CGT CGG G |
| R, GAA TTC ATC CTC GAT CCA GGC | |
| PE_PGRS6 | F, GGA TTT GGC GGG TAT TGG G |
| R, CTG CTG CTC CAC GTT GGC | |
| PE_PGRS3 | F, TCA ATG TGG TGA ACG AGC CC |
| R, CGA CGC CTA TGG AGT TCC C | |
| PE_PGRS43 | F, GAC GGC GAT CTT CCT GGG |
| R, GAG CAC AGC ATT GAA ACCG | |
| 16S rRNA | F, GCG AAC GGG TGA GTA ACA CG |
| R, TGA AAG AGG TTT ACA ACC CG |
a
F, forward primer; R, reverse primer.
b
Random primers for cDNA synthesis were the following: RPA00, NNN NNA; RPG00, NNN NNG; RPC00, NNN NNC; RPT00, NNN NNT.