TABLE 2.
Proteins found to be differentially regulatedf by exogenous cAMP in BCG grown under various conditions
| Protein spot no.a | Shaking
|
Standing
|
||||||
|---|---|---|---|---|---|---|---|---|
| High O2d
|
Low O2e
|
High O2
|
Low O2
|
|||||
| +b cAMP | −b cAMP | + cAMP | − cAMP | + cAMP | − cAMP | + cAMP | − cAMP | |
| 1 | +c | −c | + | − | − | − | + | − |
| 2 | − | − | + | − | − | − | − | − |
| 3 | − | − | + | − | − | − | + | + |
| 4 | − | − | + | − | − | − | − | − |
| 5 | + | − | + | − | − | − | + | − |
| 6 | + | − | + | − | + | − | + | − |
| 7 | + | − | + | − | − | − | − | − |
| 8 | + | − | + | − | − | − | − | − |
| 9 | − | − | + | − | + | + | + | + |
| 10 | − | − | + | − | − | − | − | − |
| 11 | + | − | + | − | − | − | − | − |
| 12 | − | − | + | − | − | − | − | − |
| 13 | + | + | + | + | + | + | + | − |
| 14 | ? | ? | + | + | + | − | + | + |
| 15 | − | − | − | − | − | − | + | − |
a
Numbers correspond to protein spots from shaking low-oxygen 2-D gel profiles (Fig 2).
b
+, 10 mM exogenous cAMP added to cultures; −, no exogenous cAMP added.
c
+, presence of protein spot under given condition; −, absence; ?, could not be determined.
d
High-oxygen conditions: 20% O2, 0% CO2.
e
Low-oxygen conditions: 1.3% O2, 5% CO2.
f
Results in boldface indicate proteins differentially regulated by exogenous cAMP.