TABLE 3.
MS data, obtained from analysis on an ion trap nano LC-nanospray mass spectrometer, for identified protein spots from 2-D GE
| Spot no.a | Protein ID | Ion strengthb | MS/MS peptide matches |
|---|---|---|---|
| 5 | GroEL2 | 1.1e6 | gi|13879960 +7 (K)DETTIVEGAGDTDAIAGR |
| 2.0e6 | gi|13879960 +4 (K)QIAFNSGLEPGVVAEK | ||
| 1.5e6 | gi|13879960 +22 (P)DLLPLLEK | ||
| 6 | Rv2971 | 9.9e5 | gi|13882834 +2 (R)LIDTAYAYGNEAAVGR |
| 2.9e6 | gi|13882834 +4 (K)YVDAWGGMIQSR | ||
| 2.0e6 | gi|13882834 +4 (K)YVDAWGGM*IQSR | ||
| 6.5e6 | gi|13882834 +2 (R)LLDNPTVTSIASEYVK | ||
| 3.1e6 | gi|13882834 +2 (R)WNLQLGNAVVVR | ||
| 7.6e6 | gi|13882834 +4 (K)YVDAWGGM*IQSR | ||
| 8.6e5 | gi|13882834 +4 (K)YVDAWGGMIQSR | ||
| 4.5e6 | gi|13882834 +2 (R)VREDPLTYAGT | ||
| 6b | fixB | gi|31619801 +3 (R)GVGSAEN#FSVVEALADSLGAAVGASR | |
| 5.7e6 | gi|31619801 +3 (R)AGAVEAEPAAGAGEQVSVEVPAAAEN#AAR | ||
| 1.1e7 | gi|31619801 +3 (R)AAVDSGYYPGQFQVGQTGK | ||
| 6.5e6 | gi|31619801 +3 (M)AEVLVLVEHAEGALK | ||
| 3.1e6 | gi|31619801 +3 (R)AAVDSGYYPGQFQVGQTGK | ||
| 1.6e5 | gi|31619801 +3 (R)AGAVEAEPAAGAGEQVSVEVPAAAEN#AAR | ||
| 1.0e7 | gi|31619801 +3 (R)AAVDSGYYPGQFQVGQTGK | ||
| 2.8e7 | gi|31619801 +3 (R)ALGEPAAVVVGVPGTAAPLVDGLK | ||
| 5.7e6 | gi|31619801 +5 (K)VSAELITAAR | ||
| 8.1e6 | gi|31619801 +3 (K)IYVAESDLVDK | ||
| 4.0e6 | gi|31619801 +5 (R)IGSGLLVDVVDVR | ||
| 1.7e6 | gi|31619801 +3 (K)TIVAVNKDEEAPIFEIADYGVVGDLFK | ||
| 1.0e6 | gi|31619801 +3 (R)EPAVAGDRPELTEATIVVAGGR | ||
| 1.1e5 | gi|31619801 +3 (R)AGAVEAEPAAGAGEQVSVEVPAAAENAAR | ||
| 1.5e6 | gi|31619801 +3 (R)EPAVAGDRPELTEATIVVAGGR | ||
| 2.8e6 | gi|31619801 +3 (R)EPAVAGDRPELTEATIVVAGGR | ||
| 1.0e6 | gi|31619801 +3 (R)ALGEPAAVVVGVPGTAAPLVDGLK | ||
| 1.3e7 | gi|31619801 (K)VAPQLTEVIK |
a
Numbers correspond to protein spots from shaking low-oxygen 2-D gel profiles (Fig 2).
b
Ion scores of ≥106 are considered significant.